Picks PCR primers and hybridization oligos and convert it to tabular emboss eprimer3.py -sequence $input1 -outfile $out_file1 -numreturn $numreturn -gcclamp $gcclamp -osize $osize -minsize $minsize -maxsize $maxsize -otm $otm -mintm $mintm -maxtm $maxtm -maxdifftm $maxdifftm -ogcpercent $ogcpercent -mingc $mingc -maxgc $maxgc -saltconc $saltconc -dnaconc $dnaconc $hybridprobe Sequences

.. class:: warningmark The input dataset needs to be sequences. ----- You can view the original documentation here_. .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/eprimer3.html