AmplifiedFragmentLengthPolymorphism

Invented by KeyGene, a Dutch biotech company based in Wageningen, Holland. The technique is now merchandised under licence agreement by PerkinElmer. Selected GeneticMarker are amplified in a PCR, which makes AFLP an easy and fast tool for strain identification in agriculture, botany, MicroBiology and AnimalBreeding.

It is a genetic mapping technique that uses selective amplification of a subset of RestrictionEnzyme digested DNA fragments to generate a unique fingerprint for a particular Genome. Commonly used for plant and microbial studies, AFLP is used for a variety of applications, such as:

• Creation of GeneticMap for new species

• Determination of relatedness among cultivars
• Establishment of linkage groups in crosses
• Genetic diversity and molecular phylogeny studies

SeeAlso http://docs.appliedbiosystems.com/pebiodocs/00113895.pdf