AmplifiedFragmentLengthPolymorphism

Invented by KeyGene, a Dutch biotech company based in Wageningen, Holland. The technique is now merchandised under licence agreement by PerkinElmer. Selected GeneticMarker are amplified in a PCR, which makes AFLP an easy and fast tool for strain identification in agriculture, botany, MicroBiology and AnimalBreeding.

It is a genetic mapping technique that uses selective amplification of a subset of RestrictionEnzyme digested DNA fragments to generate a unique fingerprint for a particular Genome. Commonly used for plant and microbial studies, AFLP is used for a variety of applications, such as:

  • Creation of GeneticMap for new species

  • Determination of relatedness among cultivars
  • Establishment of linkage groups in crosses
  • Genetic diversity and molecular phylogeny studies

SeeAlso http://docs.appliedbiosystems.com/pebiodocs/00113895.pdf

AFLP (last edited 2013-07-11 08:31:17 by 61)

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