A Protein Enzyme known to cut a half-DNA or an RNA strand at a specific sequence of bases, such as AAAACCTTT X CCC.

It means Restriction Endonuclease.

generates cohesive(sticky) ends

• EcoRI : G|AATTC - CTTAA|G
• BamHI : G|GATCC - CCTAG|G
• BglII : A|GATCT - TCTAG|A

generates flush(blunt) ends

• BalI : TGG|CCA
• SmaI : CCC|GGG

RestrictionMap or RFLP is made by RestrictionEnzyme, REBASE is its Database