Protease means the Protein(or Enzyme) which degrades (poly)peptides into shorter peptide or AminoAcids. Most frequent example is a digestion enzyme - chymotrypsin or trypsin.

Today, Protease is noticed as a drug for several Virus disease - AIDS , poliomyelitis or plant viral disease.

Protease is classified as nucleophile residue in the active site.

  1. SerineProtease

  2. AspartylProtease

  3. CysteineProtease

Cleavege site of each protease

Reagent no.

Reagent

Cleavage rule

1

Trypsin

C-term to K/R

2

Lys-C

C-term to K

3

Arg-C

C-term to R

4

Asp-N

N-term to D

5

V8-bicarb

C-term to E

6

V8-phosph

C-term to E/D

7

Chymotrypsin

C-term to F/W/Y/L/M

8

CNBr

C-term to M

SeeAlso ViralProtease

Protease (last edited 2011-11-17 08:27:04 by 211)

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