BioHackersNet|
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| A [Protein] [Enzyme] known to cut a half-[DNA] or an [RNA] strand at a specific sequence of bases, such as AAAACCTTT X CCC. | A [[Protein]] [[Enzyme]] known to cut a half-[[DNA]] or an [[RNA]] strand at a specific sequence of bases, such as AAAACCTTT X CCC. |
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| RestrictionMap or [RFLP] is made by RestrictionEnzyme, [REBASE] is its [Database] | RestrictionMap or [[RFLP]] is made by RestrictionEnzyme, [[REBASE]] is its [[Database]] |
A Protein Enzyme known to cut a half-DNA or an RNA strand at a specific sequence of bases, such as AAAACCTTT X CCC.
It means Restriction Endonuclease.
generates cohesive(sticky) ends
- EcoRI : G|AATTC - CTTAA|G
- BamHI : G|GATCC - CCTAG|G
- BglII : A|GATCT - TCTAG|A
generates flush(blunt) ends
- BalI : TGG|CCA
- SmaI : CCC|GGG
RestrictionMap or RFLP is made by RestrictionEnzyme, REBASE is its Database