BioHackersNet|
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| 논문 * [[http://www.yandell-lab.org/publications/pdf/euk_genome_annotation_review.pdf|A beginner's guide to eukaryotic genome annotation]] (Nature reviews, May 2012) |
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| * GenePrediction using GenScan, [[Orpheus]] * [[BLAST]]n in [[dbEST]] for coding region and RnaSplicing confirmation |
관련프로그램 * Pipeline: [[MAKER]], [[PASA]], [[EVM]] 관련방법들 * GenePrediction using GenScan, [[Orpheus]], [[Augustus]], [[SNAP]], [[TwinScan]], [[FGENESH]] |
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| * Gene structure determination with [[EST]], [[Protein]], [[RNA-seq]] matching |
Genome서열정보 주석달기.
어떤방법들을 통하여 Genome정보를 해석할 수 있을까.
보통은, GenePrediction --> BLAST, HMMER
관련정보
종에 따른 해석방법
Prokaryotic
TIGR에 보면 미생물 GenomeAnnotation에 관한 체계적인 프로세스가 나와있다.
Euckaryotic
논문
A beginner's guide to eukaryotic genome annotation (Nature reviews, May 2012)
관련프로그램
관련방법들
GenePrediction using GenScan, Orpheus, Augustus, SNAP, TwinScan, FGENESH
tRNA, rRNA confirmation using tRNAscan-SE
Repeat sequence confirmation using RepeatMasker
Gene structure determination with EST, Protein, RNA-seq matching
regulatory region (TranscriptionFactor) confirmation using TESS
CpG_island 탐색 using CpgPlot
ProteinStructure가 알려진 단백질에 대한 주석