What is MassSpectrometry

A MassSpectrometry works by using magnetic and electric fields to exert forces on chrged particles(ions) in a vacuum. therefore, a compound must be charged or ionized to be analyzed by a mass spectrometer.

Furthermore, the ions must be introduced in the gas phase into the vacuum system of the MassSpectrometry. This is easily done for gaseous or heat-volatile samples. However,many thermally labile analytes decompose upon heating. these kinds of samples require either desorption or desolvation methods if they are to be analysed by mass spectrometry. Although ionization and desorption/desolvation are usually separate processes, the term "ionization method" is commonly used to refer to both ionization and desorption(or desolvation)methods.

MassSpectrometry의 종류

• MALDI-TOF : MatrixAssistedLaserDesorptionIonizationTimeOfFlight

• ESI-TOF : ElectroSprayIonizationTimeOfFlight

• TandemMassSpectrometry

• FTICR

활용예

최근 Proteomics에서 ProteinIdentification 방법, ProteinProteinInteraction확인방법, PostTranslationalModification예측방법으로 널리 활용된다.

• PeptideMassFingerprint

• PostSourceDecay

• AccurateMassTag

salts and detergents에 의해서 background가 증가한다. 따라서, SDS-PAGE gels or staining reagents들이 현저하게 결과정확도를 감소시킨다. 이를 극복하는것 또한 MassSpectrometry가 풀어야할 과제이다.

관련정보

관련문서 975901444&board=PDS&command=down_load&filename=malditof발표. doc 다운로드

관련책

• ProteomeResearchMassSpectrometry

관련사이트

• ProteinProspector

• http://www.elsevier.com/homepage/sal/emas/

• METLIN

2002년 KoichiTanaka가 soft ionization에 관한 공로로 NobelPrize받다.

새로운형태의 MS